Sample suction apparatus

ABSTRACT

A sample suction apparatus includes: first through third members; a drive source; and a suction needle provided on the third member. The drive source reduces a distance between the first member and the third member to perform: a first action of shifting the first member toward the third member to contact the first member with a portion of a specimen vessel; a second action of shifting the second member together with the third member toward the first member to contact the second member with another portion of the specimen vessel so that the specimen vessel is sandwiched between the first and second members; and a third action of shifting the third member toward the first member to bring the third member close to the second member so that the suction needle is inserted in the specimen vessel.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a divisional of application Ser. No. 10/879,039,filed on Jun. 30, 2004, now U.S. Pat. No. 7,381,372 which is adivisional of application Ser. No. 09/899,178, filed Jul. 6, 2001, nowU.S. Pat. No. 6,833,113 which is based upon Japanese application No.2000-205358 filed on Jul. 6, 2000, whose priority is claimed under 35USC §119, the disclosure of which is incorporated by reference in itsentirety.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a sample suction apparatus. Inparticular it relates to a so-called piercing apparatus for piercing aplug-sealed specimen vessel with a suction needle (piercer) to suck up asample.

2. Description of Related Art

To suck up a sample such as blood out of a sealed specimen vessel,commonly employed is a method of sucking up the sample by fixing thespecimen vessel and then piercing a plug of the specimen vessel with asuction needle in the shape of an injection needle. Specifically, withrespect to the specimen vessel tilted to have its plug lower than itsbottom, a vessel supporting means is shifted to abut the bottom of thevessel and then a washing bath for washing the suction needle is shiftedto abut the plug to fix the specimen vessel. Then the suction needlepenetrates the washing bath and pierces the plug to suck up the samplecontained in the vessel (see Japanese Examined Utility Model PublicationNo. HEI 7 (1995)-3328).

In a conventional apparatus as described above, two drive sources (aircylinders) are utilized to independently move the vessel supportingmeans, the washing bath and the suction needle. If the number of thedrive source is reduced, size and production costs of the apparatus arereduced and the control thereof is simplified.

SUMMARY OF THE INVENTION

Under such circumferences, the present invention has been achieved toprovide an apparatus capable of surely fixing the specimen vessel bysandwiching it at both ends and piercing the plug with the suctionneedle, utilizing a single drive source.

The present invention provides a sample suction apparatus comprising: afirst member, a second member, and a third member capable of linearlyreciprocating along the same direction and spaced apart from each other,the second member being located between the first member and the thirdmember; a drive source provided on the third member to enlarge andreduce a distance between the first member and the third member; anelastically compressible spacer inserted between the second member andthe third member; and a suction needle provided on the third member, thesuction needle pointing to the first member, wherein the drive sourcereduces the distance between the first member and the third member toperform: a first action of shifting the first member toward the thirdmember to contact the first member with a portion of a specimen vessel;a second action of shifting the second member together with the thirdmember toward the first member to contact the second member with anotherportion of the specimen vessel so that the specimen vessel is sandwichedbetween the first and second members; and a third action of shifting thethird member toward the first member to compress the spacer to bring thethird member close to the second member so that the suction needle isinserted in the specimen vessel.

These and other objects of the present application will become morereadily apparent from the detailed description given hereinafter.However, it should be understood that the detailed description andspecific examples, while indicating preferred embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a front view for illustrating an apparatus according to anembodiment of the present invention;

FIG. 2 is a bottom view for illustrating the apparatus according to theembodiment of the present invention;

FIG. 3 is a sectional view cut along a line III-III shown in FIG. 1;

FIG. 4 is an enlarged sectional view illustrating a major part of FIG.1;

FIGS. 5( a) to 5(d) are views for illustrating an operation procedure ofthe apparatus according to the embodiment of the present invention; and

FIG. 6 is a plan view for illustrating an arrangement of a hematologyanalyzer utilizing the sample suction apparatus shown in FIG. 1.

DESCRIPTION OF THE PREFERRED EMBODIMENTS

The first, second, and third members according to the present inventionmay be sliders mounted on a linear rail. For example, commerciallyavailable RSR-ZM type sliders manufactured by THK Co., Ltd. may beutilized. In this case, a single rail, or two or three parallel railsmay be used.

It is suitable that the drive source according to the present inventionreciprocates linearly. As such a drive source, fluid pressure cylinder(e.g., an air cylinder and a hydraulic cylinder), an electric cylinderdriven by a motor and the like.

The elastically compressible spacer inserted between the second andthird members may be, for example, a compressible spring.

In the context of the present invention, the specimen vessel is a commonvessel utilized for containing samples such as blood, urea and the like.It may be a tube-shaped vessel made of glass or plastic having a bottleand an aperture sealed with a rubber plug. For example, the specimenvessel contains blood as a specimen.

The suction needle is preferably in the shape of an injection needle sothat it pierces and penetrates the plug of the specimen vessel.

To perform the first action according to the present invention, thefirst member is independently shifted to contact an end of the specimenvessel and then the second and third members are shifted after the firstmember has contacted the end.

This is carried out by utilizing a stopper as the first member and abiasing member for biasing the third member toward a direction oppositeto the first member. The biasing member may be a compressible spring.

The second action according to the present invention is performed insuch a manner that the third member pushes the second member toward thefirst member via the spacer after the first member has contacted thespecimen vessel and stopped.

The third action is performed in such a manner that the third membercompresses the spacer after the second member has contacted the specimenvessel and stopped.

According to the present invention, the second member may be providedwith a washing bath for washing the suction needle so that the suctionneedle is washed before or after the insertion to the specimen vessel.

The apparatus of the present invention may further comprise a sensor fordetecting completion of the second action. With the sensor, all theactions may be recovered from a state where the specimen vessel does notexist between the first and second members or a state where the specimenvessel is not properly introduced.

Embodiment

Hereinafter, the present invention will be detailed by way of anembodiment with reference to the drawings, but the invention is notlimited thereto.

FIG. 1 is a front view for illustrating a sample suction apparatusaccording to an embodiment of the present invention, FIG. 2 is a bottomview for illustrating the same and FIG. 3 is a sectional view cut alonga line III-III shown in FIG. 1.

As shown in these figures, a single rail 2 is fitted to a flap portionprovided in a lower part of a substrate 1. Sliders 3, 4, and 5 areslidably mounted on the rail 2, which are capable of linearlyreciprocating along the rail 2, respectively. On the slider 5, an aircylinder 7, a suction needle (hereinafter referred to as a piercer) 8,and a spring supporting part 9 are mounted via a mounting part 6.

On the slider 4, a washing bath 11 and a spring supporting part 12 aremounted via a mounting part 10. On the slider 3, a supporting part 14 ismounted via a mounting part 13. The mounting part 13 is connected with adistal end of a piston rod 7 a of the air cylinder 7 via a connectionpart 13 a.

The piercer 8 is mounted on the mounting part 6 via a piercer supportingpart 8 a. The supporting part 14 includes a conical hollow 14 a to abuton a bottom 15 a of a specimen vessel 15. The specimen vessel 15contains a liquid sample such as blood to be measured and is sealed witha rubber plug 15 b.

The washing bath 11 is a hollow box having a drain 16 at the bottomthereof and small pores 17 and 18 through which the piercer 8penetrates. Stoppers 19 and 20 are mounted on the substrate 1. Thestopper 19 restricts the movement of the air cylinder 7 along thedirection of an arrow C shown in FIG. 1. The stopper 20 allows thepiston rod 7 a penetrate therethrough and restricts the movement of theconnection part 13 a, i.e., the mounting part 13, along the direction ofthe arrow C. A compressible spring 21 is wound about the piston rod 7 abetween the stopper 20 and the air cylinder 7 for biasing the aircylinder toward the direction of the arrow C.

Further, as shown in FIG. 2, a compressible spring 22 is supported byspring supporting parts 9 and 12 and biases the spring supporting parts9 and 12 to keep them apart from each other. An adjusting screw 23(FIGS. 1 and 3) is provided on the mounting part 6 so that a tip of theadjusting screw 23 abuts on the mounting part 10 to adjust a positionalrelationship between a tip of the piercer 8 and the washing bath 11(during washing of the piercer 8).

The supporting part 14 is mounted on the mounting part 13 via a mountingplate 24 and biased toward the direction of the arrow C by acompressible spring 25 installed therein as shown in FIG. 4. Further, aphotointerrupter 26 is mounted on the mounting plate 24. Thephotointerrupter 26 is actuated by a tip 14 b of the supporting part 14when the supporting part 14 moves along the direction of an arrow B.

The relationship in spring coefficient between the compressible springs21, 22, and 25 is established as follows:

Compressible spring 22>compressible spring 25

Compressible spring 22>compressible spring 21

An operation procedure of the thus constructed apparatus will bedetailed with reference to FIGS. 5( a) to 5(d).

As shown in FIG. 5( a), a specimen vessel 15 held by a hand clipper (notshown) is introduced between the supporting part 14 and the washing bath11 of the sample suction apparatus which is in a starting state.

Then, the air cylinder 7 is actuated to drag the piston rod 7 a alongthe direction of the arrow C. Since the compressible spring 21 isprovided between the stopper 20 and the air cylinder 7 for biasing themto keep away from each other, the air cylinder 7 keeps contact with thestopper 19 without moving and the mounting part 13 is shifted along thedirection of the arrow C to abut on the stopper 20 as shown in FIG. 5(b). Thus, the supporting part 14 contacts the bottom 15 a of thespecimen vessel 15.

As the air cylinder 7 further drags the piston rod 7 a along thedirection of the arrow C, the compressible spring 21 is compressed sincethe distal end of the piston rod 7 a is fixed with the stopper 20, andthen the air cylinder 7 and the mounting part 6 are shifted along thedirection of the arrow B as shown in FIG. 5( c).

Simultaneously with the above, the mounting part 10 is shifted along thedirection of the arrow B by force applied toward the arrow B via thecompressible spring 22. Accordingly, the washing bath 11 presses therubber plug 15 b of the specimen vessel 15 in the direction of the arrowB. Thus, the specimen vessel 15 is sandwiched between the supportingpart 14 and the washing bath 11, and at the same time, the supportingpart 14 compresses the compressible spring 25 (FIG. 4) and the tip 14 bthereof actuates the photointerrupter 26.

Then, as the air cylinder 7 further drags the piston rod 7 a along thedirection of the arrow C, the air cylinder 7 and the mounting part 6 areshifted toward the direction of the arrow B as shown in FIG. 5( d). Atthis stage, the washing bath 11 is abutting on the specimen vessel 15and does not move, so that the compressible spring 22 is compressed andthe tip of the piercer 8 passes through the small pore 18 and penetratesthe rubber plug 15 b to enter the specimen vessel 15.

Then, as shown in FIG. 1, a suction apparatus 28 connected with thepiercer 8 is actuated to suck up the sample from the specimen vessel 15via the piercer 8. A predetermined amount of the sucked sample iscollected by a quantitative measurement part 27.

After the suction of the sample has been completed, the air cylinder 7pushes out the piston rod toward the direction of the arrow B. As aresult, the actions carried out in the order of FIGS. 5( a) to 5(d) areperformed in an opposite order. When the sample suction apparatusreturns to the state shown in FIG. 5( a), washing solution is suppliedfrom a washing apparatus 29 (FIG. 1) to the washing bath 11 via thepiercer 8.

The washing solution is also supplied toward the piercer 8 from a cap(not shown) formed in the washing bath 11 so that the inner and outerwalls of the piercer 8 are washed. Waste solution is then dischargedfrom the drain 16.

Thus, the suction of the sample via the piercer 8 and the washing of thepiercer 8 are performed.

In the case where the photointerrupter 26 is not actuated while shiftingfrom the state of FIG. 5( b) to the state of FIG. 5( c), a controlsection (not shown) judges that the specimen vessel 15 is absent orintroduced improperly between the supporting part 14 and the washingbath 11, and then returns the sample suction apparatus to the state ofFIG. 5( a).

For easy suction of the sample from the specimen vessel 15 via thepiercer 8, it is preferable to incline the specimen vessel 15 by 30 to45° with respect to a horizontal line so that the bottom 15 a is heldhigher than the rubber plug 15 b. The inclination is carried out byadjusting the degree of angle of the substrate 1 with respect to thehorizontal line. In this case, the rail 2 is also inclined and thus theair cylinder 7 is always biased toward the stopper 19 by its weight, sothat the compressible spring 21 is omitted.

FIG. 6 is a view for illustrating an arrangement of a hematologyanalyzer provided with the sample suction apparatus of the presentinvention.

Racks 126 each have the shape of a test tube stand capable of carryingten specimen vessels 15.

The racks 126 each carrying the specimen vessels 15 are arranged along avertical line on a conveyer 112. Then, all the racks 126 move toward adirection of an arrow D indicated in FIG. 6 and the rack 126 at thefront is transferred to a conveyer 110 located on the left hand of theconveyer 112.

A hematology analyzer 118 is provided above the conveyer 110. The rack126 transferred on the conveyer 110 is stopped immediately below astirrer 111 of the hematology analyzer 118.

After a specimen in one of the specimen vessels 15 at the front isstirred with the stirrer 111, the rack 126 moves by a predetermineddistance (an arrangement pitch among the specimen vessels 15 on the rack126). The specimen vessel 15 immediately after subjected to stirring isheld by a hand clipper 117 and withdrawn from the rack 126 to suck upthe specimen (blood) by a suction apparatus 115, and then returned tothe rack 126. The sucked specimen is quantified by a quantitativemeasurement part 113 and analyzed by an analysis part 114. While thesuction apparatus 115 sucks up the specimen from the preceding specimenvessel, the stirrer 111 stirs a specimen contained in the next specimenvessel 15.

Thus, the rack 126 moves intermittently by the arrangement pitch of thespecimen vessels 15. After the stirring and the suction of the specimenin the ten specimen vessels 15 (all the specimen vessels carried on therack 126) are completed, the rack 126 is transferred along a directionof an arrow E. Then the specimen vessel 15 at the front of the followingrack 126 is subjected to stirring. The racks 126 that went through thestirring and the suction of the specimen in the ten specimen vessels arefinally collected at a rack collecting part 116 and arranged along adirection of an arrow F.

In the hematology analyzer 118, the sample suction apparatus shown inFIG. 1 is utilized as the suction apparatus 115.

According to the present invention, the action of fixing the specimenvessel and the action of inserting the suction needle in the specimenvessel are performed with a single drive source, so that the structureand the control of the sample suction apparatus are simplified.

1. A sample suction apparatus for aspirating a specimen from a specimenvessel sealed with a plug by using a suction needle, comprising: asubstrate; a rail which is supported on the substrate; an air cylindercomprising a cylinder part and a piston rod movable to the cylinder; afirst member which is connected with the piston rod and is movable alongthe rail; a second member comprising a suction needle, wherein thesecond member is connected with the cylinder part and is movable alongthe rail; and a third member which is arranged between the first memberand the second member, and has a through-hole for inserting the suctionneedle, wherein the first member supports a bottom of the specimenvessel and the third member supports the plug of the specimen vessel. 2.The sample suction apparatus of claim 1, wherein a distal end of thepiston rod is connected with the first member.
 3. The sample suctionapparatus of claim 1, wherein the rail is inclined at 30 degree to 45degree with respect to a horizontal line.
 4. The sample suctionapparatus of claim 3, wherein a support position of the first memberwhich supports the bottom of the specimen vessel is higher than asupport position of the third member which supports the plug of thespecimen vessel.
 5. The sample suction apparatus of claim 4, wherein thesubstrate comprises a stopper for restricting movement of the aircylinder by its weight.
 6. The sample suction apparatus of claim 1,wherein the piston rod moves to the cylinder part so that the suctionneedle penetrates the plug of the specimen vessel after the first andthird members support the specimen vessel.
 7. The sample suctionapparatus of claim 1, wherein the first member comprises a supportingpart having a conical hollow.
 8. A hematology analyzer comprising thesample suction apparatus of claim 1 and analyzing part for analyzing thesucked specimen by the sample sucking apparatus.
 9. A sample suctionapparatus for aspirating a specimen from a specimen vessel sealed with aplug by using a suction needle, comprising: a substrate; a rail which issupported on the substrate; an air cylinder comprising a cylinder partand a piston rod; a first member which is connected with the piston rodand is movable along the rail; a second member comprising a suctionneedle, wherein the second member is connected with the cylinder partand is movable along the rail; a third member which is arranged betweenthe first member and the second member, and has a through-hole forinserting the suction needle; and a resilient member arranged betweenthe second and third members, wherein the first member supports a bottomof the specimen vessel and the third member supports the plug of thespecimen vessel.
 10. The sample suction apparatus of claim 9, whereinthe resilient member comprises a spring.
 11. The sample suctionapparatus of claim 10, wherein the spring is a compressible spring. 12.The sample suction apparatus of claim 10, wherein one end of the springis supported by the second member and other end of the spring issupported by the third member.
 13. The sample suction apparatus of claim9, wherein a distal end of the piston rod is connected with the firstmember.
 14. The sample suction apparatus of claim 9, wherein the firstmember comprises a supporting part having a conical hollow.
 15. Ahematology analyzer comprising the sample suction apparatus of claim 9and analyzing part for analyzing the sucked specimen by the samplesucking apparatus.
 16. A sample suction apparatus for aspirating aspecimen from a specimen vessel sealed with a plug by using a suctionneedle, comprising: an air cylinder comprising a cylinder part and apiston rod; a first member which is connected with the piston rod and ismovable with the piston rod; a second member comprising a suctionneedle, wherein the second member is connected with the cylinder partand is movable with the cylinder part; and a third member which isarranged between the first member and the second member, and comprises awashing bath for washing the suction needle, wherein the first membersupports a bottom of the specimen vessel and the third member supportsthe plug of the specimen vessel.
 17. The sample suction apparatus ofclaim 16, comprising a washing solution supplier for supplying a washingsolution to the suction needle, wherein the washing bath comprises adrain for discharging the washing solution.
 18. The sample suctionapparatus of claim 16, further comprising a spring, wherein one end ofthe spring is supported by the second member and other end of the springis supported by the third member.
 19. The sample suction apparatus ofclaim 16, wherein a distal end of the piston rod is connected with thefirst member.
 20. The sample suction apparatus of claim 16, wherein thefirst member comprises a supporting part having a conical hollow.